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曾严 / 教授


在美国SUNY Health Science Center at Brooklyn 和 Washington University 获得硕士、博士学位,在美国 Duke University,Virginia Commonwealth University,和 University of Minnesota 工作,于2014年到南农工作。研究哺乳动物microRNA的加工机制、 microRNA表达的调控,和microRNA在各种环境、条件下的表达与功能。另外,我们还在验证复杂生物系统中的相对特异性假说。我们利用体外试管、细胞培养和动物等模型, 采用和开发了各种技术,包括生物化学、遗传学、分子生物学、细胞生物学、药理学、基因组学、生物信息学、系统生物学等手段, 进行研究。已发表论文和书籍章节50篇,论文被引用约4000次, H指数28。


Journal Review: BBA - Gene Regulatory Mechanisms, BBA-Molecular Cell Research, Biochimie, BioMed Research International, BioTechniques, BMC Genomics, Cellular and Molecular Life Sciences, Cellular Physiology and Biochemistry, Comprehensive Physiology, Current Genomics, Drug Discovery Today, Encyclopedia of Molecular Cell Biology and Molecular Medicine, Evidence-Based Complementary and Alternative Medicine, Expert Opinion on Biology Therapy, FEBS J., FEBS Lett., Genomics Proteomics and Bioinformatics, J. Biotechnology, J. Cardiovascular Translational Res., J. Diabetes Research, J. Oncology, J. Visualized Experiments, Microarrays, Molecular Systems Biology, Mutation Research, Nucleic Acids Research, Recent Patents on DNAG and Gene Sequence, RNA, Toxicology Letters, and Toxins.


American Association for the Advancement of Science
RNA Society
State of Minnesota Board of Pharmacy


1. Zeng, Y., Weiss, C., Yao, T. T., Huang, J., Siconolfi-Baez, L., Hsu, P., and Rushbrook, J. I. (1995) Isocitrate dehydrogenase from bovine heart: primary structure of subunit 3/4. Biochem. J. 310: 507-516.

2. Zeng, Y., Forbes, K. C., Wu, Z., Moreno, S., Piwnica-Worms, H., and Enoch, T. (1998) Replication checkpoint requires phosphorylation of the phosphatase Cdc25 by Cds1 or Chk1. Nature 395: 507-510.

3. Zeng, Y. and Piwnica-Worms, H. (1999) DNA damage and replication checkpoints in fission yeast require nuclear exclusion of Cdc25 via 14-3-3 binding. Mol. Cell. Biol. 19: 7410-7419.

4. Weiss, C., Zeng, Y., Huang, J., Sobocka, M. B., and Rushbrook, J. I. (2000) Bovine NAD+ dependent isocitrate dehydrogenase: alternative splicing and tissue dependent expression of subunit 1. Biochemistry 39: 1807-1816.

5. Zeng, Y., Russell, R.A., Erlwein, O., Cullen, B.R., and McClure, M.O. (2001) The R region found in the Human Foamy Virus long terminal repeat is critical for both Gag and Pol protein expression. J. Virology 75: 6817-6824.

6. Wiegand, H. L., Coburn, G. A., Zeng, Y., Kang, Y., Bogerd, H. P., and Cullen, B. R. (2001) Formation of Tap/NXT1 heterodimers activates Tap-dependent nuclear mRNA export by enhancing recruitment to nuclear pore complexes. Mol. Cell. Biol. 22: 245-256.

7. Zeng, Y., Wagner, E. J., and Cullen, B. R. (2002) Both natural and designed microRNAs can inhibit the expression of cognate mRNAs when expressed in human cells. Mol. Cell 9: 1327-1333.

8. Zeng, Y., and Cullen, B. R. (2002) RNA interference in human cells is restricted to the cytoplasm. RNA 7: 855-860.

9. Zeng, Y., and Cullen, B. R. (2003) Sequence requirements for microRNA processing and function in human cells. RNA 9: 112-123.

10. Zeng, Y., Yi, R., and Cullen, B. R. (2003) microRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms. Proc. Natl. Acad. Sci. U.S.A. 100: 9779-9784.

11. Zeng, Y., and Cullen, B. R. (2004) Structural requirements for pre-microRNA binding and nuclear export by Exportin 5. Nucleic Acids Research 32: 4776-4785.

12. Zeng, Y., Cai, X., and Cullen, B. R. (2005) Using RNA polymerase II to transcribe artificial microRNAs. Methods in Enzymology 392: 371-380.

13. Zeng, Y., Yi, R., and Cullen, B. R. (2005) Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha. EMBO J. 24: 138-148.

14. Zeng, Y., and Cullen, B. R. (2005) Efficient processing of primary microRNA hairpins by Drosha requires flanking nonstructured RNA sequences. J. Biol. Chem. 280: 27595-27603.

15. Zeng, Y., and Cullen, B. R. (2006) Recognition and cleavage of primary microRNA transcripts. Methods Mol. Biol. 342: 49-56.

16. Zeng, Y., and Cullen, B.R. (2006) The biogenesis and function of microRNAs. In Gene Expression and Regulation, J. Ma ed. (Higher Education Press/Springer), pp. 481-492.

17. Zeng, Y. (2006) Principles of microRNA production and maturation. Oncogene 25: 6156-6162.

18. Zeng, Y., Sankala, H., Zhang, X., and Graves, P (2008) Phosphorylation of Argonaute2 at serine 387 facilitates its localization to processing bodies. Biochem. J. 413: 429-436.

19. Kalscheuer, S., Zhang, X., Upadhyaya, P., and Zeng, Y. (2008) Differential expression of microRNAs in early stage neoplastic transformation in the lungs of F344 rats chronically treated with the tobacco carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. Carcinogenesis 29: 2394–2399.

20. Kren, B. T., Wong, P. Y.-P., Sarver, A., Zhang, X., Zeng, Y., and Steer, C. J. (2009) microRNAs identified in highly purified liver-derived mitochondria may play a role in apoptosis. RNA Biol. 6: 65-72.

21. Zhang, X., Xu, W, Tan, J., and Zeng, Y. (2009) Stripping custom microRNA microarrays and the lessons learned about probe:slide interactions. Anal. Biochem. 386: 222-227.

22. Zhang, X., Graves, P. R., and Zeng, Y. (2009) Stable Argonaute2 overexpression differentially regulates microRNA production. Biochimica Biophysica Acta 1789: 153-159.

23. Zeng, Y. (2009) Regulation of the mammalian nervous system by microRNAs. Mol. Phar. 75: 259-264.

24. Zeng, Y. (2009) RNAi in mammalian cells. In RNA Interference: Methods for Plants and Animals, T. Doran and C. Helliwell eds. (CABI International), pp 205-227

25. Cunningham, J. M., Oberg, A. L., Borralho, P. M., Kren, B. T., French, A. J., Wang, L., Bot, B. M., Morlan, B. W., Silverstein, K. A. T., Staggs, R., Zeng, Y., Lamblin, A.-F., Hilker, C. A., Fan, J.-B., Steer, C. J., and Thibodeau, S N. (2009) Evaluation of a New High-Dimensional miRNA Profiling Platform. BMC Medical Genomics 2: 57.

26. Kren, B. T., Wong, P. Y.-P., Shiota, A., Zhang, X., Zeng, Y., and Steer, C. J. (2009) Polysome trafficking of transcripts and microRNAs in regenerating liver after partial hepatectomy. Am J Physiol Gastrointest Liver Physiol 297: 1181-1192.

27. Zheng, H., Zeng, Y., Zhang, X., Chu, J., Loh, H. H., and Law, P.-Y. (2010) μ-Opioid Receptor Agonists Differentially Regulate the Expression of miR-190 and NeuroD. Mol. Phar. 77: 102-109.

28. Melkamu, T., Zhang, X., Tan, J., Zeng, Y., and Kassie, F. (2010) Alteration of microRNA expression in vinyl-carbamate-induced mouse lung tumors and modulation by the chemopreventive agent indole-3-carbinol. Carcinogenesis 31: 252-258.

29. Zhang, X., and Zeng, Y. (2010) Regulation of mammalian microRNA expression. J. Cardiovascular Translational Res. 3: 197-203.

30. Park, C. W., Zeng, Y., and Steer, C. J. (2010) Human DNA methyltransferase 3a does not associate with microRNAs in the regulation of DNA methylation. J. Cardiovascular Translational Res. 3: 290-295.

31. Zheng, H., Zeng, Y., Chu, J., Kam, A. Y. F., Loh, H., and Law, P.-Y. (2010) Modulations of NeuroD Activity Contribute to the Differential Effects of Morphine and Fentanyl on Dendritic Spine Stability. J. Neurosci. 30: 8102-8110.

32. Chen, Y., Zhou, H., Sarver, A., Zeng, Y., Roy-Chowdhury, J., Steer, C., Sahin, M. B. (2010) Hepatic differentiation of liver derived progenitor cells and microRNA analysis of their in vitro differentiation. Liver Transplantation 16: 1086-1097.

33. Zheng, H., Chu, J., Zeng, Y., Loh, H. H., and Law, P.-Y. (2010) Yin Yang 1 Phosphorylation Contributes to the Differential Effects of mu-Opioid Receptor Agonists on MicroRNA-190 Expression . J. Biol. Chem 285: 21994-22002.
34. Zhang, X., and Zeng, Y. (2010) The terminal loop region controls microRNA processing by Drosha and Dicer. Nucl. Acids Res. 38: 7689-7697.

35. Castro, R. E., Ferreira, D. M. S., Zhang, X., Borralho, P. M., Sarver, A. L., Zeng, Y., Steer, C. J., Kren, B. T., and Rodrigues, C. M. P. (2010) Identification of microRNAs during rat liver regeneration after partial hepatectomy and modulation by ursodeoxycholic acid. American Journal of Physiology - Gastrointestinal and Liver Physiology 299: G887-897.

36. Park, C. W., Zeng, Y., Zhang, X., Subramanian, S., and Steer, C. J. (2010) Mature microRNAs identified in highly purified nuclei from HCT116 colon cancer cells. RNA Biology 7:606-614. PMID: 20864815.

37. Ghosh, G., Subramanian, I. V., Adhikari, N., Zhang, X., Joshi, H. P., Basi, D. L., Chandrashekhar, Y. S., Hall, J. L., Roy, S., Zeng, Y., and Ramakrishnan, S. (2010) Hypoxia-induced microRNA-424 expression in endothelial cells regulates HIF-1? isoforms by targeting CUL2 and promotes angiogenesis. J. Clin. Invest. 120: 4141–4154.

38. Lu, C., Huang, X., Zhang, X., Roensch, K., Cao, Q., Nakayama, K., Blazar, B. R., Zeng, Y., and Zhou, Z. (2011) miR-221 and miR-155 regulate human dendritic cell development, apoptosis and IL-12 production through targeting of p27kip1, KPC1 and SOCS-1. Blood 117: 4293-4303.

39. Shu, J., Kren, B. T., Xia, Z., Wong, P. Y., Li, L., Hanse, E. A., Min, M. X., Li, B., Albrecht, J. H., Zeng, Y., Subramanian, S., and Steer, C. J. (2011) Genome-wide microRNA downregulation as a negative feedback mechanism in the early phases of liver regeneration. Hepatology 54: 609-619.

40. Feng, Y., Zhang X., Song, Q., Li, T., and Zeng, Y. (2011) Drosha processing controls the specificity and efficiency of global microRNA expression. Biochimica Biophysica Acta, 1809: 700-707.

41. Zhang, X., and Zeng, Y. (2011) Performing custom microRNA microarray experiments. Journal of Visualized Experiments 56: e3250.

42. Zeng, Y. (2011) The functional consequences and implications of relative substrate specificity in complex biochemical systems. Front. Systems Biology 2: 65.

43. Feng, Y., Zhang, X., Graves, P., and Zeng, Y. (2012) A comprehensive analysis of precursor microRNA cleavage by human Dicer. RNA 18: 2083-2092.

44. Graves, P., and Zeng, Y. (2012) Biogenesis of mammalian microRNAs: a global view. Genomics Proteomics & Bioinformatics 10: 239-245.

45. Zhang, X., Graves, P., and Zeng, Y. (2013) Overexpression of human Argonaute2 inhibits cell and tumor growth. Biochimica Biophysica Acta-General Subjects 1830: 2553–2561.

46. Banerjee, S., Meng, J., Das, S., Krishnan, A., Haworth, J., Charboneau, R., Zeng, Y., Ramakrishnan, S., and Roy, S. (2013) Morphine induced exacerbation of sepsis is mediated by tempering endotoxin tolerance through modulation of miR-146a. Scientific Reports 3: 1977.

47. Zheng, Y., Tan, D., Chen, Z., Hu, C., Mao, Z. J., Singleton, T. P., Zeng, Y., Shao, X., and Yin, B. (2014) A Non-genetic approach to labelling acute myeloid leukemia and bone marrow cells with quantum dots. J. Nanosci. Nanotechnol. 14: 4014-4021.

48. Zeng, Y. (2014) Relative Specificity: all substrates are not created equal. Genomics Proteomics & Bioinformatics 12: 1-7.

49. Joshi, H. P., Subramanian, I. V., Ghosh, G., Rupaimoole, R., ,Evans, C., Saluja, M., Jing, Y., Cristina, I., Zeng, Y., Shah, V. H., Sood, A. K., and Ramakrishnan, S. (2014) Dynamin 2 along with its intron-associated miR-199a reciprocally regulate HIF-1 and metastasis. Proc. Natl. Acad. Sci. U.S.A. 111 : 5331–5336.

50. Hu, C., Chen, Z, Zhao, W., Wei, L, Zheng, Y., He, C., Zeng, Y., and Yin, B. (2014) Vesicular Stomatitis Virus G Glycoprotein and ATRA Enhanced Bystander Killing of Chemoresistant Leukemic Cells by Herpes Simplex Virus Thymidine Kinase/Ganciclovir. Biomol Ther. 22: 114-121.

51. Li, L., Feng, H., Da, Q., Jiang, H., Chen, L., Xie, L., Huang, Q., Xiong, H., Luo, F., Kang, L., Zeng, Y., Hu, H., Hou, W., and Feng, Y. (2016) Expression of HIV-encoded microRNA-TAR and its inhibitory effect on viral replication in human primary macrophages. Arch Virol. 161(5): 1115-1123.




1. 国家自然科学基金面上项目:31570843、microRNA 靶基因识别的系统生物学、2016/01-2017/12、30万元、在研、主持。
2. 国家自然科学基金面上项目:81471940、HIV-1通过调控miR-146a逃逸巨噬细胞抗病毒天然免疫的分子机制、2015/01-2018/12、80万元、在研、参加。
3. 国家自然科学基金面上项目:81271818、vmiR-TAR 与Tat 相互作用在HIV-1 病毒潜伏感染中的作用机制研究、2013/01-2016/12、80万元、在研、参加。