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徐冬青 / 副教授

个人简介

徐冬青

E-mail: dqxu at njau.edu.cn

office 生科楼C7019

1991.9-1995.7 南京农业大学动物资环学院微生物专业,获学士学位

1995.9-1998.7 南京农业大学动物资环学院微生物专业,获硕士学位

1998.9-2002.1 中科院上海植物生理生态研究所微生物专业,获博士学位

2002.5-2005.4 美国德克萨斯大学MD Anderson 癌症研究中心分子遗传系,博士后

研究方向

微生物酶的结构与功能,微生物分子遗传学,微生物生理生化


教学情况

教学

本科生:基础微生物学,微生物学实验,微生物生理学,蛋白质化学

研究生:微生物生理及遗传学进展,高级微生物学


科研情况

论文

Zhang Ya, Qiu Shenshen, Jia Shanshan, Xu Dongqing, Ran Tingting*, Wang Weiwu* Crystal structure of the sensor domain of BaeS from Serratia marcescens FS14.  Proteins. 2017 Sep;85(9):1784-1790. doi: 10.1002/prot.25326.

Zhang F, Wei Q, Tong H, Xu D, Wang W, Ran T.* Crystal structure of MBP-PigG fusion protein and the essential function of PigG in the prodigiosin biosynthetic pathway in Serratia marcescens FS14.  International Journal of Biological Macromolecules.  99 (2017) 394–400

Xu Dongqing, Zhou Jiale, Lou Xiangdi, He Jianhua, Ran Tingting*, Wang Weiwu*.  Myroilysin is a New Bacterial Member of the M12A Family of Metzincin Metallopeptidases and Activated by a Cysteine-switch Mechanism.   J Biol Chem. 2017 Mar 31;292(13):5195-5206. doi: 10.1074/jbc.M116.758110. Epub 2017 Feb 9.

Dongxia Wu, Pengpeng Li, Jiale Zhou, Meijing Gao, Xiangdi Lou, Tingting Ran, Shuwen Wu, Weiwu Wang, Dongqing Xu*, Identification of a toxic serralysin family protease with unique thermostable property from S. marcescens FS14, International Journal of Biological Macromolecules 93 (2016) 98–106

Wei Q#, Ran T#, Ma C, He J, Xu D*, Wang W*.Crystal Structure and Function of PqqF in the Pyrroloquinoline Quinone Biosynthetic Pathway. J Biol Chem. 2016 VOL. 291, NO. 30, pp. 15575–15587, July 22, 2016

Wu D, Ran T, Wang W, Xu D*. Structure of a thermostable serralysin from Serratia sp. FS14 at 1.1 ? resolution. Acta Crystallogr F Struct Biol Commun. 2016 Jan; 72(Pt 1):10-5

Zhou H, Li P, Wu D, Ran T, Wang W, Xu D*. EheA from Exiguobacterium sp. yc3 is a novel thermostable DNase belonging to HNH endonuclease superfamily. FEMS Microbiol Lett. 2015 Dec; 362(24):fnv204.

Xu D, Gao Y, Wang P, Ran T, Wang W*, Presence of an amino acid residue at position 619 required for the function of YidC in Rhodobacter sphaeroides. Biochem Biophys Res Commun. 2015 Oct 16;466(2):267-71

Li P, Kwok AH, Jiang J, Ran T, Xu D, Wang W, Leung FC*Comparative genome analyses of Serratia marcescens FS14 reveals its high antagonistic potential. PLoS One. 2015 Apr 9; 10(4):e0123061.

Ran T, Gao M, Wei Q, He J, Tang L, Wang W, Xu D*.Expression, crystallization and preliminary crystallographic data analysis of VioD, a hydroxylase in the violacein-biosynthesis pathway. Acta Crystallogr F Struct Biol Commun. 2015 Feb; 71(Pt 2):149-52.

王 峰,任春梅,季英华,缪 倩,姚恋秋,周益军,徐冬青*,程兆榜*,黄瓜绿斑驳花叶病毒海南分离物基因组测定与毒源分析,201440675-81

Han N, Ran T, Lou X, Gao Y, He J, Tang L, Xu D* and Wang W*. Expression, crystallization and preliminary crystallographic data analysis of PigI, a putative L-prolyl-AMP ligase from the prodigiosin synthetic pathway in Serratia., Acta Cryst. 2014, F70, 624627

Lou X#, Ran T#, Han N, Gao Y, He J, Tang L, Xu D*, Wang W*. Crystal structure of the catalytic domain of PigE: A transaminase involved in the biosynthesis of 2-methyl-3-n-amyl-pyrrole (MAP) from Serratia sp. FS14. Biochem Biophys Res Commun. 2014 Apr 25; 447(1):178-83. doi: 10.1016/j.bbrc.2014.03.125.

Ran T, Gao Y, Marsh M, Zhu W, Wang M, Mao X, Xu L, Xu D*, Wang W*. Crystal structures of Cg1458 reveal a catalytic lid domain and a common catalytic mechanism for FAH family. Biochem J. 2013 Jan 1;449(1):51-60.

Zhu W, Lan Y, Lou X, Han N, Ran T, Xu L, Xu D, Wang W*Isolation of proteorhodopsin-bearing bacterium JL-3 from fresh water and characterization of the proteorhodopsin. FEMS Microbiol Lett. 2013 Jul; 344(1):10-7.

Liu S, Ran T, Shen X, Xu L, Wang W, Xu D* Expression, crystallization and preliminary crystallographic data analysis of PigF, an O-methyltransferase from the prodigiosin-synthetic pathway in Serratia. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Aug; 68(Pt 8):898-901.

Ran T, Wang Y, Xu D, Wang W*. Expression, purification, crystallization and preliminary crystallographic analysis of Cg1458: a novel oxaloacetate decarboxylase from Corynebacterium glutamicum. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Aug 1;67(Pt 8):968-70.

郑朝成,王长林,付玲,王伟武,徐冬青*, 一株产耐高温DNA酶和蛋白水解酶的沙雷氏菌FS14Serratia sp.FS14)的分离与鉴定。微生物学通报,38(2):228~236

Xu D, W Mattox, Identification of a splicing enhancer in MLH1 using COMPARE a new assay for determination of relative RNA splicing efficiencies, Hum Mol Genet. 2006, 15(2):329-36

Xu D, Wu Y, Isolation and Analysis of hupR Gene Required for the Expression of Hydrogenase in Rhodobacter sphaeroides, 生物化学和生物物理学报 2001,33(6), 607-614

徐冬青, 吴永强, 光合菌Rhodobacter sphaeroides 601 hupT基因的克隆与功能分析, 植物生理学报, 2001, 27(6), 509-514

徐冬青,陈永萱, 检测棉花黄萎病菌的间接ELISA法建立与应用, 南京农业大学学报, 2000, vol.23, 105-108

课题

一种分泌型耐高温双功能酶的催化活性分子机制及其在沙雷氏菌中生理功能的研究(2012.1-2014.12),国家自然基金青年基金,主持

结核分支杆菌S2P的体外表达,纯化与特性研究及对毒力因子调控途径的初步研究,(2009.1-2011.12),教育部博士点新教师基金,主持

光驱动质子泵变形菌视紫红质(Proteorhodopsins)的晶体结构及质子转运机理的解析 (2012.1-2015.12),国家自然基金,参加

膜蛋白位点2蛋白酶的晶体结构解析及功能的研究(2012.8-2014.7),中央高校基本科研业务费,参加

YidC对浑球红细菌光合系统膜生物发生及能量转换的研究(2010.1-2012.12),教育部重点项目,参加

Proteorhodopsin的分子结构及其质子转运功能的研究(2008.1-2010.12),国家自然基金,参加