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崔中利 / 教授

个人简介

简介/introduction

博士生导师,江苏省 “333高层次人才培养工程”首批中青年科学技术带头人,“青蓝工程”中青年学术带头人培养对象,入选教育部“新世纪优秀人才资助计划”项目,江苏省杰出青年基金资助,国家科技进步二等奖获得者(第二获奖人)。主要从事微生物技术在农产品安全生产中的应用方面的研究,主持完成国家“863”计划项目、国家自然科学基金项目多项。2005-2007年在日本京都大学进行了蛋白质晶体结构解析方面的研究工作,熟悉蛋白质晶体培养、X-衍射数据分析析,解析了海藻糖降解途径中的鼠李糖苷酶的结构。发表论文100余篇,其中在appl. Environ. Microbiol., J molecular boil. 等杂志上发表SCI论文40余篇。

主要研究方向:


1 生物降解与生物转化
2 酶资源与蛋白质工程
3 土传病害真菌防治


Main Research Feilds
Microbial degradation of environmental pollutants
Microorganisms play important roles in the removal of organic pollutants in the environment. Degradative bacteria are the keys factor in the efficient bioremediation of organic pollutants. We focus on the development of microbiological techniques for bioremediation of chemical pesticides pollution and clarifying the microbial mechanisms during the degradation. A large number of strains that could degrade pesticides and herbicides have been isolated in our laboratory. Most of these strains could completely mineralize the target chemicals. For example, Pseudomonas putida DLL-1 was able to utilize parathion and methyl parathion as sole carbon sources. Genes involved in degradation of methyl parathion was cloned from strain DLL-1. Methyl parathion hydrolase Mph was a novel phosphotriesterase reported by our lab. It was completely different from Oph from Flavobacterium sp. ATCC27551. Strains with high capacity towards hexachlorohexane, atrazine, 3,5,6-Trichloro-2-pyridinol, and acetochlor were isolated. Field experiments showed that degradative strains can effectively remove pesticides residues in soils.


Resources of novel biocatalyst and Crystallographic of enzymes of potential industrial application

Enzymes are important catalyst in biotransformation of important chemical intermediates in pharmaceutical, brewery, biofuel, and fine chemicals industry. Thus enzymes with high catalytic ability or special substrate affinity are important resources. Biodegradation is closely related to biotransformation. We are seeking to screen enzymes involved in biodegradation for potential application in biotransformation of valuable products. We also try to use other methods to screen novel enzyme such as metagenomic method. We have set up methods to isolate bacterial cells from soil and extract high molecular DNA fragment for BAC library construction. A BAC library for DNA extracted from red soil was constructed. A novel CMC-cellulase was screened from this library. We are now trying to sequence part of this library with 454 prosequencing to primarily analysis the characteristics of bacterial genomes in red soil. In future, we hope to screen enzymes from different ecological environment by metagenoics.

Enzymes involved in organic pollutants degradation are of special interest to protein crystallographer. These enzymes are different from enzymes involved in the common life process. They are evolved for the metabolism of special chemicals, especially artificially synthesized compounds. We focus on the solving of the 3-D structure of enzymes involved in biodegradation. We have solved the structure of a novel 1,2,4-trihydroxybenezen dioxygenase. Crystals of p-nitrophenol 4-monooxygenase and a novel CMC-cellulase diffracting to high resolution were screened, which could diffract to 1.9 ?. Structures could give hints of the catalytic mechanisms of these enzymes and also lay the foundation for rational design of high effective degradative enzymes for bioremediation.


Biocontrol of plant fungus pathogens by Myxobacteria

Myxobacteria are a group of special gram-negative bacteria, which can move on solid surface without of flagellum. These bacteria can predate other microorganisms in soils. Strain EGB is a myxobacteria strain isolated in my lab which shows strong ability in predating pathogenic fungi. Our aim is to clarify the the predation mechanisms of strain EGB on fungi and its application in pathogenic fungi biocontrol.


教学情况

博士生:现代微生物研究技术进展


科研情况

获得的项目资助/fundings

国家重点基础研究发展计划(973计划):作物高产高效的土壤微生物区系特征及其调控,2015CB150502, 80万。
国家自然科学基金:新型外膜蛋白型β-1,6-葡聚糖酶GluM性质及其在珊瑚球菌EGB菌株捕 食植物病原真菌中的作用研究,31570059,77万
国家自然基金:Pseudomonas putida DLL-E4对硝基苯酚降解中的温敏与转录调控机制,31270095,82万
科技部“十二五”科技支撑:循环农业系统污染物减控关键技术研究。2012BAD14B02-03,50万。
江苏省杰出青年基金:微生物降解有机污染物的分子与结构生物学研究,BK2012029,100万
农业部948项目:提高农药残留微生物降解性能的超级通道技术的引进与应用,2011-Z21,60万。
国家转基因专项:微生物来源防虫抗除草剂基因在转基因水稻种的功能,2009.7-2011.7,子课题主持。2009ZX08009-056B,82万
国家自然科学基金:农用化学物质微生物降解关键酶的蛋白质晶体学研究,2007.12—2010.12,主持。3077033
国家高技术研究计划(863)重大专项子课题:农药污染环境微生物资源的开发利用,2007.2-2010.1,主持。2007AA021304,81万
教育部新世纪优秀人才:环境微生物学,2006.1--2010.12,主持。NCET-06-0490.
国家自然科学基金:3077033有机磷水解酶结构与功能的初步研究,2003.1—2005.12,主持
国家高技术发展研究计划(863计划):高效安全的农药残留降解菌剂研制及其在绿色农产品上的应用,崔中利,50万元,2004.3—2005.10,项目编号:2004AA246070
国家高技术发展研究计划(863计划):高效安全的农药残留降解菌剂研制及其在绿色农产品上的应用,崔中利,75万元,2001.1—2003.3,项目编号:2001AA246071
粘细菌多功能β-1,3-葡聚糖酶的结构和催化机理研究,BK20140687, 20万, 2014.7-2017.6
国家青年基金:粘细菌新型β-1,3-葡聚糖酶的催化机制研究,31400056, 23万,2015.1.1-2017.12.31

发表论文/publications

1. Zhoukun Li , Jiale Wu, Biying Zhang, Fei Wang, Xianfeng Ye, Yan Huang, Qiang Huang and Zhongli Cui. AmyM, a Novel Maltohexaose-Forming _-Amylase from Corallococcus sp. Strain EGB. Applied and Environmental Microbiology, 2015,81(6):1977-1987
2. Weiliang Dong, Qiongzhen Chen, Ying Hou, Shuhuan Li, Kai Zhuang, Fei Huang, Jie Zhou, Zhoukun Li, Jue Wang, Lei Fu, Zhengguang Zhang, Yan Huang, Fei Wang and Zhongli Cui. Metabolic Pathway Involved in 2-Methyl-6-Ethylaniline Degradation by Sphingobium sp. Strain MEA3-1 and Cloning of the Novel Flavin-Dependent Monooxygenase System meaBA. Appl. Environ. Microbiol. 2015 ,81 (24): 8254-8264
3. Weiliang Dong, Ying Hou, b, Xuedong Xi, Fei Wang, Zhoukun Li, Xianfeng Ye, Yan Huang, Zhongli Cui. Biodegradation of fenoxaprop-ethyl by an enriched consortium and its proposed metabolic pathway. International Biodeterioration & Biodegradation, 2015, 97, 159–167
4. Yan Xin, Zhibin Sun, Qiongzhen Chen, Jue Wang, Yicheng Wang, Linfeng Luogong, Shuhuan Li, Weiliang Dong, Zhongli Cui, Yan Huang. Purification and Characterization of a Novel Extracellular Thermostable Alkaline Protease from Streptomyces sp. M30. Journal of Microbiology and Biotechnology , 2015, 25, 1944-1953.
5. Zhibin Sun, Pingping Liu, Guangyan Cheng, Biying Zhang, Weiliang Dong, Xingli Su, Yan Huang, Yi Kong, Zhongli Cui, A ?brinolytic protease AfeE from Streptomyces sp.CC5,with potent thrombolytic activity in a mouse model, International Journal of Biological Macromolecules, http://dx.doi.org/10.1016/j.ijbiomac.2015.12.059
6. Ying Hou, Shuhuan Li, Weiliang Dong, Yi Yuan, Yicheng Wang, Wenjing Shen, Jingquan Li, Zhongli Cui. Community structure of a propanil-degrading consortium and the metabolic pathway of Microbacterium sp. strain T4-7. International Biodeterioration & Biodegradation 2015, 105, 80–89.
7. Jiale Wu, Bingjie Xia, Zhoukun Li, Xianfeng Ye, Qiongzhen Chen, Weiliang Dong, Jie Zhou, Yan Huang, Zhongli Cui. Molecular cloning and characterization of a novel GH13 saccharifying α-amylase AmyC from Corallococcus sp. EGB. Starch/St?rke 2015, 67, 810–819.
8. Zhoukun Li , Kai Ji , Weiliang Dong , Xianfeng Ye, Jiale Wu , Jie Zhou , Fei Wang , Qiongzhen Chen, Lei Fu , Shuhuan Li , Yan Huanga , Zhongli Cui. Cloning, heterologous expression, and enzymatic characterization 4 of a novel glucoamylase GlucaM from Corallococcus sp. strain EGB. Protein Expression and Puri?cation xxx (2015) xxx–xxx.
9. Zhibin Sun, Weihao Lu, Pingping Liu, Hui Wang, Yan Huang, Yuguo Zhao, Yi Kong, Zhongli Cui. Isolation and characterization of a proteinaceous α-amylase inhibitor AAI-CC5 from Streptomyces sp. CC5, and its gene cloning and expression. Antonie van Leeuwenhoek, 2015, 107(2),345-356
10. Zhibin Sun, Ding Li, Pingping Liu, Wenhui Wang, Kai Ji, Yan Huang, Zhongli Cui. A novel l-asparaginase from Aquabacterium sp. A7-Y with self-cleavage activation. Antonie van Leeuwenhoek, 2015, 10.1007/s10482-015-0614-0
11. Fei Wang, Jie Zhou, Zhoukun Li, Weiliang Dong, Ying Hou, Yan Huang and Zhongli Cui. Cytochrome P450 System EthBAD involved in theN-deethoxymethylation of Acetochlor byRhodococcus sp. T3-1. Applied and Environmental Microbiology, 2015,81(6):2182-2188.
12. Baiping Zhao, Xiude Hua, Fei Wang, Weiliang Dong, Zhoukun Li, Yu Yang, Zhongli Cui, Minghua Wang. Biodegradation of propyzamide by Comamonas testosteroni W1 and cloning of the propyzamide hydrolase gene camH. Bioresource Technology 179 (2015) 144–149.
13. Weiliang Dong, Ying Hou , Shuhuan Li, Fei Wang , Jie Zhou , Zhoukun Li , Yicheng Wang, Fei Huang, Lei Fu, Yan Huang, Zhongli Cui. Purification, cloning, expression, and biochemical characterization of a monofunctional catalase, KatP, from Pigmentiphaga sp. DL-8. Protein Expression and Purification 108 (2015) 54–61
14. Zhibin Sun, Yan Huang, Yanzhuo Wang, Yuguo Zhao, Zhongli Cui. Potassium hydroxide-ethylene diamine tetraacetic acid method for the rapid preparation of small-scale PCR template DNA from actinobacteria. Molecular Genetics, Microbiology and Virology, 2014, 29, 42-46.
15. Weiliang Dong, Ying Hou, Xuedong Xi, Fei Wang, Zhoukun Li, , Xianfeng Ye, Yan Huang,Zhongli Cui. Biodegradation of fenoxaprop-ethyl by an enriched consortium and its proposed metabolic pathway. International Biodeterioration & Biodegradation, 2015, 97, 159–167.
16. Fei Wang, Ying Hou, Jie Zhou, Zhoukun Li, Yan Huang, Zhongli Cui. Purification of an amide hydrolase DamH from Delftia sp. T3-6 and its gene cloning, expression, and biochemical characterization. Applied Microbiology and Biotechnology, 2014, 98, 7491-7499.
17. Zhibin Sun, Weihao Lu, Pingping Liu, Hui Wang, Yan Huang, Yuguo Zhao, Yi Kong, Zhongli Cui. Isolation and characterization of a proteinaceous α-amylase inhibitor AAI-CC5 from Streptomyces sp. CC5, and its gene cloning and expression. Antonie van Leeuwenhoek, 2014, DOI 10.1007/s10482-014-0333-y.
18. Xiaojun Hu, Jue Wang, Fei Wang, Qiongzhen Chen, Yan Huang, Zhongli Cui. Complete Genome Sequence of the p-Nitrophenol-Degrading Bacterium Pseudomonas putida DLL-E4. Genome Announc. 2014, 2, e00596-14.
19. Hou, Ying; Dong, Weiliang; Wang, Fei; Li, Jingquan; Shen, Wenjing; Li, Yongfeng; Cui, Zhongli. Degradation of acetochlor by a bacterial consortium of Rhodococcus sp.T3-1, Delftia sp.T3-6 and Sphingobium sp.MEA3-1. Letters in Applied Microbiology, 2014, 323(2):196-203.
20. Jingquan Li, Yan Huang, Ying Hou, Xiangmin Li, Hui Cao, Zhongli Cui?. Novel Gene Clusters and Metabolic Pathway Involved in 3,5,6-Trichloro-2-Pyridinol Degradation by Ralstoniasp.T6. Appl. Environ. Microbiol. 2013, 79(23):7445-53.
21. Xuedong Xi, Weiliang Dong, Jun Zhang, Yan Huang, Zhongli Cui?. Taonella mepensis gen. nov., sp. nov., a member of the family Rhodospirillaceae isolated from activated sludge. Int J Syst Evol Microbiol. 2013, 63(7):2472-2476.
22. Xuedong Xi, Jing Fan, Ying Hou, Jinhua Gu, Wenjing Shen, Zhoukun Li, Zhongli Cui?. Characterization of three cryptic plasmids from Lactobacillus plantarum G63 that was isolated from Chinese pickle, Plasmid, 2013, 70(3): 321-328.
23. Ying Hou, Jian Tao, Wenjing Shen, Juan Liu, Jingquan Li, Yongfeng Li, Hui Cao & Zhongli Cui. Isolation of the fenoxaprop-ethyl (FE)-degrading bacterium Rhodococcus sp. T1, and cloning of FE hydrolase gene feh. FEMS Microbiology letters. 2011,323(2):196-203.

申请专利/patents

ZL 200510022544.9 一种毒死蜱农药残留降解菌及其生产的菌剂,6/6
ZL 200510022551.9 一种高效聚磷菌及其生产的菌剂,4/4
ZL201010018298.0 纤维素水解酶β-1,4葡聚糖内切酶基因
ZL201010506356 一种rRNA嵌合启动子及含有该嵌合启动子的表达载体
ZL201110189139 精噁唑禾草灵水解酯酶基因、含有该基因的工程菌及其应用
ZL 201310041368 一株产聚β-羟基丁酸酯细菌及其应用
ZL 201310028459 一种珊瑚球菌及其应用
ZL201310043628 一种α-淀粉酶及其基因、含有该基因的工程菌及其应用
申请号 201410713567 一种新型中温α-淀粉酶的应用
申请号 201510816987.9 一种新的β-1,6-葡聚糖酶及其编码基因和应用

获奖/Awards

教育部技术发明奖,土壤农药污染微生物修复技术的研究与应用,二等,2007,第二获奖人(李顺鹏,崔中利,何健,洪青,蒋建东,管晓进)
国家科技进步奖,农药残留微生物降解技术的研究与应用,二等,2005,第二获奖人(10)(李顺鹏、崔中利、沈 标、刘 智、何 健、杨兴明、王新华、张瑞福、蒋建东、洪 青),2005-J-201-2-03-D01