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微生物学系

王伟武 教授
 
姓名:王伟武
职称:教授
联系电话:025-
EMAIL:wwwang at njau.edu.cn
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一、 主要学历及研究经历
1990.9-1994.7 安徽农业技术师范学院兽医系,获学士学位
1994.9-1997.7 南京农业大学动物医学院动物传染病学专业,获硕士学位
1997.9-2001.7 中科院上海植物生理生态研究所微生物学专业,获博士学位
2001.10-2005.4 美国德克萨斯大学休斯顿医学院微生物系和膜蛋白研究中心,博士后
2005-2007 瑞士Paul Scherrer Institute生物大分子系膜蛋白结构生物学研究组,博士后

2007  至今      南京农业大学生命科学学院

二、主要研究方向
蛋白质三维晶体结构和功能,微生物分子遗传学

三、近年来主要研究工作
主要研究微生物中重要膜蛋白,酶蛋白的三维结构和功能

国家自然科学基金项目:
Proteorhodopsin的分子结构及其质子转运功能的研究(2008.1-2010.12)
霍乱弧菌草酰乙酸脱羧酶晶体结构与其脱羧和钠离子跨膜转运功能体现 (2010.1-2012.12)  
光驱动质子泵变形菌视紫红质(Proteorhodopsins)的晶体结构及质子转运机理的解析(2012.1-2015.12)
教育部项目:
YidC对浑球红细菌光合系统膜发生及能量转化的影响(2010-2012)
Proteorhodopsin的晶体结构及功能研究

四、近年来发表的主要论文

 Han N, Ran T, Lou X, Gao Y, He J, Tang L, Xu D* and Wang W*. Expression, crystallization and preliminary crystallographic data analysis of PigI, a putative L-prolyl-AMP ligase from the prodigiosin synthetic pathway in Serratia. Acta Cryst. (2014). F70, 624–627

Lou X#, Ran T#, Han N, Gao Y, He J, Tang L, Xu D*, Wang W*. Crystal structure of the catalytic domain of PigE: A transaminase involved in the biosynthesis of 2-methyl-3-n-amyl-pyrrole (MAP) from Serratia sp. FS14. Biochem Biophys Res Commun. 2014 Apr 2. pii: S0006-291X(14)00585-3. doi: 10.1016/j.bbrc.2014.03.125.

Ran T#, Ozorowski G#, Gao Y#, Sineshchekov O.A, Wang W*, Spudich J.L*, Luecke H* Cross-protomer interaction with the photoactive site in oligomeric proteorhodopsin complexes. Acta Crystallogr D Biol Crystallogr. 2013 Oct;69(Pt 10):1965-80. doi: 10.1107/S0907444913017575. Epub 2013 Sep 20.(封面文章)  

Zhu W, Lan Y, Lou X, Han N, Ran T, Xu L, Xu D, Wang WW*  Isolation of proteorhodopsin-bearing bacterium JL-3 from fresh water and characterization of the proteorhodopsin. FEMS Microbiol Lett. 2013 Jul;344(1):10-7. doi: 10.1111/1574-6968.12144.

Ran T, Gao Y, Marsh M, Zhu W, Wang M, Mao X, Xu L, Xu D*, Wang W*. Crystal structures of Cg1458 reveal a catalytic lid domain and a common catalytic mechanism for FAH family. Biochem J. 2013 Jan 1;449(1):51-60. doi: 10.1042/BJ20120913.

 Liu S, Ran T, Shen X, Xu L, Wang W, Xu D* Expression, crystallization and preliminary crystallographic data analysis of PigF, an O-methyltransferase from the prodigiosin-synthetic pathway in Serratia. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Aug;68(Pt 8):898-901.

 Wang N, Wang M, Gao Y, Ran T, Lan Y, Wang J, Xu L*, Wang W*. Crystallization and preliminary X-ray crystallographic analysis of a blue-light-absorbing proteorhodopsin. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Mar 1;68(Pt 3):281-3. Epub 2012 Feb 22.

 Ran T, Wang Y, Xu D, Wang W*. Expression, purification, crystallization and preliminary crystallographic analysis of Cg1458: a novel oxaloacetate decarboxylase from Corynebacterium glutamicum. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Aug 1;67(Pt 8):968-70. Epub 2011 Jul 27.

 Li Y, Zhu H, Zeng X, Fan J, Qian X, Wang S, Wang Z, Sun Y, Wang X, Wang W, Ju D. Suppression of autophagy enhanced growth inhibition and apoptosis of interferon-β in human glioma cells. Mol Neurobiol. 2013 Jun;47(3):1000-10. doi: 10.1007/s12035-013-8403-0. Epub 2013 Jan 18. 

Wu J, Li J, Mao X, Wang W, Cheng Z, Zhou Y, Zhou X, Tao X. Viroplasm protein P9-1 of Rice black-streaked dwarf virus preferentially binds to single-stranded RNA in its octamer form, and the central interior structure formed by this octamer constitutes the major RNA binding site. J Virol. 2013 Dec;87(23):12885-99. doi: 10.1128/JVI.02264-13. Epub 2013 Sep 25.

郑朝成,王长林,付玲,王伟武,徐冬青*。一株产耐高温DNA酶和蛋白水解酶的沙雷氏菌FS14Serratia sp.FS14)的分离与鉴定。微生物学通报,2011, 38(2):228~236

 齐立辉,郑新艳,冉婷婷,徐朗莱,王伟武*。 沙门氏菌草酰乙酸脱羧酶在大肠杆菌中的表达、纯化及其生化鉴定。 南京农业大学学报。201033( 4) : 81-86

 Studer R*, Dahinden P*, Wang WW*, etc. Crystal Structure of the Carboxyltransferase Domain of the Oxaloacetate Decarboxylase Na(+) Pump from Vibrio cholerae. J Mol Biol. 2007,367(2):547-57.

 Wang Wei-wu, Gao jin, Jiao Rui-sheng, Zhao Guoping, Jiang Wei-hong. A novel two-component system amrB-amkB involved in the regulation of central carbohydrate metabolism in rifamycin SV-producing Amycolatopsis mediterranei U32. Curr Microbiol. 2004 Jan;48(1):14-9.

 Mao X, Wang W, Jiang W, Zhao GP. His23beta and Glu455beta of the Pseudomonas sp. 130 glutaryl-7-amino cephalosporanic acid acylase are crucially important for efficient autoproteolysis and enzymatic catalysis. Protein J. 2004 23(3):197-204

Man, D*, Wang WW*, Sabihe G, Aravind L, Post AF, Massana R, Spudich EN, spudich JL , Beja O. Diversification and spectral tuning in marine proteorhodopsins. EMBO J. 2003,22, 1725–1731 (封面文章,*these authors contribute equally)

 Wang, WW, Sineshchekov OA, Spudich EN, spudich JL. Spectroscopic and photochemical characterization of a deep ocean proteorhodopsin. J Biol Chem. 2003 Sep 5;278(36):33985-91.

Wang W, Gao J, Chiao J, Zhao G, Jiang W. A novel two-component system amrB-amkB involved in the regulation of central carbohydrate metabolism in rifamycin SV-producing Amycolatopsis mediterranei U32. Curr Microbiol. 2004 Jan;48(1):14-9.

 Wang Wei-wu, Zhang Weiwen, Chen Hui, Chiao Juishen,Zhao Guoping, Jiang Wei-hong. Molecular and biochemical characterization of a novel two-component signal transduction system, amrA- amkA, involved in rifamycin SV production in Amycolatopsis mediterranei U32. Arch Microbiol. 2002,178(5):376-86.

 Wang Wei-wu,Zhang Weiwen, Lu Jie,Yang Yunliu, Jiao Rui-sheng, Zhao Guoping, Jiang Wei-hong. MoeA, an enzyme in the molybdopterin synthesis pathway, is required for rifamycin SV production in Amycolatopsis mediterranei U32. Appl Microbiol Biotechnol. 2002,60(1-2):139-46.

五、联系方式
Telephone: 025-(O);
E-mail: wwwang@njau.edu.cn

office: 生科楼c7019

 

 
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